Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (Biotin)

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£953.00

SKU:: J8009-BIOTIN
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal of human JAM-1 protein
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Weitere Details:: The junctional adhesion molecule-1 (JAM-1) is an integral membrane protein that colocalizes with tight junction molecules occludin, ZO-1, and cingulin.1 JAM-1 has been characterized as a member of the immunoglobulin (Ig) superfamily and is composed of an extracellular domain, a single-pass transmembrane region, and a short cytoplasmic tail.2 JAM-1 is localized at tight junctions in endothelial and epithelial cells and its sub-cellular distribution is modulated by inflammatory cytokines.2 JAM-1 is also expressed on circulating leukocytes and platelets as well as in lymphoid.2 Most claudins as well as JAM-1 end with the amino acid Val at their C-terminal ends, suggesting that these COOH termini directly bind to PDZ domains.3 JAM-1 appears to be a single copy gene, which is constitutively expressed on various tissues and cells, and may be involved in cell-to-cell adhesion through homophilic interaction.4 JAM-1 was detected in human intestine cell lines T84, CaCO2 and HT29, in the lung cell line A 549, and the liver cell line HepG2.5 Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1–3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice