J2475-01A-ML490
JNK1 (c-Jun N-terminal Kinase 1, JNK1A2, JNK, JNK21B1/2, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1) (MaxLight 490)
Größe
£914.00
- SKU:
- J2475-01A-ML490
- Reinheit:
- Purified by Protein A chromatography
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant GST-fusion protein of JNK1
- Klon:
- 4A2G12
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Weitere Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The mitogen-activated protein kinase (MAP) kinase family includes ERK, JNK and p38 kinase. The pathway, regulated by (MAP) kinase family, involves a broad array of cellular responses that include survival, cell proliferation, and apoptosis. The JNK signal transduction pathway is activated in response to environmental stress and by engagement of several classes of cell surface receptors including cytokine receptors, serpentine receptors and receptor tyrosine kinases. Although JNK is located in both the cytoplasm and the nucleus of quiescent cells, activation of JNK is associated with accumulation of JNK in the nucleus. In the NGF (nerve growth factor)-differentiated PC-12 cells, activation of the ERK signaling pathway promotes cell survival but stimulation of the JNK and p38 signaling pathways contributes to cell death. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilution: Immunofluorescence: 5-10ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice