I8850-40

Isocitrate Dehydrogenase [NADP], Cytoplasmic (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD)

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£696.00

SKU:: I8850-40
Reinheit:: Purified by ammonium sulfate precipitation.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa122-152 from the center region of human IDH1.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Weitere Details:: IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.