Iron Responsive Element Binding Protein 2 (IREB2, IREBP2, ACO3, FLJ23381, Iron Regulatory Protein 2, IRP2, IRP2AD)

Größe

£626.00

SKU:: I8615-75B
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). Species Sequence Homology: primate and rat, 94%
Format:: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen). Applications: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Immunofluorescence (IC): 1:500 Immunohistochemistry (Frozen/paraffin): 1:200-1;500. Heat Induced antigen retrieval with 10mM sodium citrate buffer, pH 9.0 and endogenous peroxidase quenching with peroxide block is needed for paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice