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I8449-07C

IP10 (Interferon gamma Inducible Protein, CXCL10) (Biotin)

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£629.00

SKU:
I8449-07C
Reinheit:
Purified by affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to mouse IP-10, expressed in E. coli.
Format:
Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.05mg/ml.
Weitere Details:
IP-10 is a CXC chemokine that signals through the CXCR3 receptor. IP-10 selectively chemoattracts Th1 lymphocytes and monocytes, and inhibits cytokine-stimulated hematopoietic progenitor cell proliferation. Additionally, it is angiostatic and mitogenic for vascular smooth muscle cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Detection): 0.25-1ug/ml (100ul/well). Used in conjunction with I8449-05A as a caputure antbody detects 0.2-0.4ng/well of recombinant mouse IP10 Western Blot: 0.1-0.2ug/ml; Detection limit is 1.5-3ng/lane for recombinant mouse IP10 under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Versandbedingungen:
Blue Ice