I8443-18F
Interleukin 22 (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)
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£1,042.00
- SKU:
- I8443-18F
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- E. coli-derived recombinant mouse IL-22 (aa 34-179; UniProt #Q9JJY9).
- Klon:
- 8H107
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Weitere Details:
- Interleukin 22 (IL-22), also called IL-10-related T cell-derived inducible factor or IL-TIF, is a secreted cytokine that contributes to the inflammatory response in vivo. IL-22 is expressed in T cells and mast cells in response to IL-9 or lipopolysaccharide. IL-22 monomers bind a class II cytokine receptor that is a heterodimer of CRF2-9 (IL-22 Ra1) and CRF2-4 (IL-10 Rb). Mouse IL-22 is a 179 aa protein with a 33 aa signal peptide, three N-glycosylation sites and two intramolecular disulfide bonds. The mature protein shows 78% and 90% aa identity with human and rat IL-22, respectively. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml. Detection limit for recombinant mouse IL-22 is ~1ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-5 x10e5 cells in a total reaction mixture not exceeding 200ul. For intracellular staining with PMA/ionomycin- stimulated EL-4 cells to detect IL-22, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Secondary antibody such as anti-rat IgG conjugated to a fluorochrome can be used. Direct ELISA: 0.5-1ug/ml. Detection limit for recombinant mouse IL-22 is ~2.5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice