I8443-18A
Interleukin 22 (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)
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£668.00
- SKU:
- I8443-18A
- Reinheit:
- Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse IL-22, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile ddH20.
- Weitere Details:
- Interleukin-22 (IL-22) is a regulatory cytokine and member of the IL-10 family, secreted by activated T cells, which signals through a class II cytokine receptor system consisting of IL-22R and IL-10R-beta/CRF2-4. IL-22 has been shown to act as an inhibitor of the production of IL-4 by Th2 cells, up-regulate the production of acute phase reactants in the pancreas and liver, and to be up-regulated in intestinal inflammation associated with Crohn’s disease. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilution: Sandwich ELISA: 0.5-2ug/ml. In conjunction with Biotinated anti-mouse IL-22 as a detection antibody detects at least 2000-4000pg/ml of recombinant mouse IL-22. Western Blot: 0.1 -0.2ug/ml. The detection limit is 1.5-3ng/lane under either reducing or non-reducing conditions Neutralization: 2ug/ml yeilds one half maximal inhibition (ND50) of the biological activity of mouse IL-22 (50ng/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O . Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice