Interleukin 18 (Interferon-gamma-inducing factor, Iboctadekin, IFN-gamma-inducing factor, IGIF, IL-18, IL1F4, IL-1g, IL-1 gamma, Interferon gamma-inducing factor, Interleukin-18, Interleukin-1 gamma, MGC12320)

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£642.00

SKU:: I8439-95S
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-NPPDNIKDTKSDI, from the internal region of human Interleukin 18 (NP_001553.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: IL-18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL-12 has been shown to inhibit IL-4-dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL-18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL-18 increases production of competent HIV. IL-18 is related to the IL-1 family. One of the IL-18 receptor chains is the IL-1 receptor-related protein, a member of the IL-1R family. Like IL-1, IL-18 is first synthesized as a leaderless precursor requiring the IL-1 converting enzyme (ICE) for cleavage into an active molecule. IL-18 may play a role in tissue injury in inflammatory reactions. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 (detection limit dilution) Western Blot: 1-3ug/ml. A band of ~23kD was observed in human placenta lysates and in lysates of cell line HeLa. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice