I8432-29D
Interleukin 10R, beta (IL-10 R-beta, Interleukin 10 Receptor beta, IL-10R2, CD210b, CRF2-4) (APC)
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£812.00
- SKU:
- I8432-29D
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met20-Ser220 of human Interleukin 10 receptor beta expressed in NS0 cells.
- Klon:
- 11n101
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- The IL-10 receptor consists of two chains, a ligand binding IL-10 Ra (IL-10 R1) chain and a signaling chain known as IL-10 Rb (IL-10 R2).1, 2 The human IL-10 Rb chain is a 325aa protein with 69% homology to its mouse counterpart.3 Stimulation of cells by IL-10 activates both Jak1 and Tyk2 kinases.2 The IL-10 Rb chain is also a component of the recently identified IL-22 receptor.4 Both chains of the IL-10 R are expressed on lymphocytes, monocytes, and neutrophils, with the IL-10 Rb chain expressed at higher levels than the IL-10 Ra chain in most cases.5 IL-10 signaling can affect a variety of immune functions, including inhibition of pro-inflammatory cytokine synthesis and regulation of the growth and function of B cells and antigen-presenting cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 human peripheral blood monocytes Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells Balb/c mice. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice