I8432-25A
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
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£403.00
- SKU:
- I8432-25A
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- COS-expressed human IL-10
- Klon:
- JES3-9D7
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
- Weitere Details:
- Applications: ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified antibody should be paired with biotinylated monoclonal antibody as the detection antibody, with purified recombinant human IL-10 as the standard. Immunofluorescence/Flow Cytometry: The antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control. Western Blotting: The purified antibody is also useful for Western blotting applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100 ng/lane of recombinant human IL-10 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice