Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF) (Biotin)

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£479.00

SKU:: I8432-17
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IL-10 expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.1-1mg/ml. Labeled with Biotin.
Weitere Details:: The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (sandwich): 0.25-1ug/ml (using 100µl/well) in conjunction with Anti Human IL-10 (I8432-15A) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant human IL-10. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible development reagents the detection limit for recombinant human IL-10 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice