I7663-97D
Interleukin 33 (IL-33)
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£320.00
- SKU:
- I7663-97D
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C (-20°C Glycerol)
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human IL-33 (112-270aa) purified from E. coli.
- Klon:
- 8k278
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40%.
- Weitere Details:
- Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kD and C-terminal fragment of 18kD by cleavage of caspase-1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:250 Western Blot: 1:250-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-33 protein. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice