I7663-33H
IL2RG (Cytokine Receptor Common Subunit gamma, Interleukin-2 Receptor Subunit gamma, IL-2 Receptor Subunit gamma, IL-2R Subunit gamma, IL-2RG, p64, CD132)
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£856.00
- SKU:
- I7663-33H
- Reinheit:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.10EU/ug (LAL)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu23-Asn254 from human IL2RG expressed in S. frugiperda insect ovarian cell line
- Klon:
- 11n93
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- The g chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 Rg is a 369aa protein consisting of a 22aa signal sequence, a 232aa extracellular domain, a 29aa transmembrane domain and an 86aa cytoplasmic domain. Although IL-2 Rg by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the g chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 Rg has been designated the common g chain (gc). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 Rg gene. Applications: Suitable for use in Flow Cytometry, Neutralization, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 using human peripheral blood lymphocytes Neutralization: Measured by its ability to neutralize IL2-induced proliferation in the MO7e human megakaryocytic leukemic cell line (ref 3). The Neutralization Dose (ND50) is typically 0.08-0.4ug/ml in the presence of 200ng/ml of recombinatn human IL-2 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice