I7661-39D
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
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£991.00
- SKU:
- I7661-39D
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human umbilical vein EC
- Klon:
- 6A561 (BV7)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA.
- Weitere Details:
- Beta-1 integrin is a ubiquitously expressed ~89 kD type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple Beta-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Functional Studies. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50 Stains the extracellular domain of beta-1 integrin. Fix HUVEC cells in 4% paraformaldehyde before staining. Functional Studies: Functions as an inhibiting antibody. Functionally tested by adhesion assay (1), chemotaxis (2), patch clamp analysis (3) and neurotoxicity induced apoptosis (4). Optimal dilutions to be determined by the researcher. Positive Control: HT-29 colon carcinoma, Endothetlial cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice