I7661-17D

Insulin-like Growth Factor Binding Protein 7 (IGF Binding Protein 7, IGFBP7, IGFBP-7, IGFBP-7v, IBP-7, FSTL2, IGFBPRP1, IGFBP-rP1, MAC25 Protein, MAC25, Prostacyclin-stimulating Factor, PGI2-stimulating Factor, PSF, Tumor-derived Adhesion Factor, TAF)

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£639.00

SKU:: I7661-17D
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IGFBP7, expressed in E coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile ddH2O.
Weitere Details:: Insulinlike growth factor binding protein7, also known as mac25, a 256 aa secreted protein and member of a growing family of IGFBPs which regulate the biological activities and distribution of the insulinlike growth factors (IGFs), through binding to IGFI and IGFII. IGFBP7 is widely expressed throughout normal tissues, but is also expressed in many tumor types at an increased or reduced level, and acts as a suppressor of breast and prostate cancer cell lines. IGFBP7 inhibits IGFinduced differentiation of skeletal muscle (myogenesis) and has implications in bone formation, by its possible affects on the actions of parathyroid hormone (PTH) and cortisol. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with I7661-17A1, as a detection antibody allows the detection of at least 0.2-0.4ng/well of recombinant human IGFBP7. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice