I7660-07
Insulin, Recombinant, Mouse (INS)
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£372.00
- SKU:
- I7660-07
- Molekulargewicht:
- 10
- Reinheit:
- ≥95%
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Weitere Details:
- Recombinant protein corresponding to aa25-54 of mouse Insulin-B linked by polypeptide linker (GGGGS) to aa90-110 of mouse Insulin A with a N-terminal His-Tag, expressed in E. coli. UniProt/Accession: P01326. Amino Acid Sequence: FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSIC SLYQLENYCN Preditcted Molecular Weight: 10.3kD Accurate Molecular Weight: 10kD (SDS-PAGE reducing conditions) Predicted Isoelectric Point: 7.1 Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Versandbedingungen:
- Blue Ice