I5000
Interleukin 1 Receptor AcP (IL1RacP, IL-1 Receptor Accessory Protein)
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£655.00
- SKU:
- I5000
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 22 amino acids near the carboxy terminus of human Interleukin 1 Receptor AcP. Epitope: within the last 50 amino acids Species Sequence Homology: rat; 91%, mouse; 86%.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Detects a band at ~63kD with whole HeLa lysates Immunocytochemistry: 5ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: HeLa whole lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice