IgG, F(ab’)2 (Biotin)

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£524.00

SKU:: I1904-45C
Reinheit:: Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Human IgG F(ab’)2 fragment
Format:: Biotin conjugated. Lyophilized in 0.01 M Sodium Phosphate, 0.15M sodium chloride, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Thimerosal
Weitere Details:: Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule Application(s): Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilution(s): This product has been assayed against 1.0 ?g of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:30,000 to 1:120,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice