IgG, H&L (AP)

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£593.00

SKU:: I1903-21M
Reinheit:: Purified by immunoaffinity chromatography
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Horse IgG whole molecule. Label: Alkaline Phosphatase (Calf Intestine) (MW140,000 daltons)
Format:: Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide.
Weitere Details:: Applications: Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (standard capture): 1:500-1:1500, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.