I1900-06N

Immunoglobulin E (IgE)

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£505.00

SKU:: I1900-06N
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Purified human IgE
Klon:: 8L344 (4H10)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Weitere Details:: Immunoglobulin E (IgE) is a 180kD soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. Applications: Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.