Hypoxia Inducible Factor 1a (HIF-1a) (APC)

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£780.00

SKU:: H9807-04-APC
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Fusion protein corresponding to the internal region, aa432-528, of human HIF-1a
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Allophycocyanin (APC).
Weitere Details:: Hypoxia Inducible Factor 1 (HIF-1) is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. Applications: Suitable for use in FLISA, Western Blot, Gel Shift Assays, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Versandbedingungen:: Blue Ice