HIF1A, CT (Hypoxia Inducible Factor 1 alpha, Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS Protein 1, MOP1, PAS Domain-containing Protein 8, PASD8)

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£704.00

SKU:: H9804-01E
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human HIF1A at C-terminal.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). Applications: Suitable for use in Western Blot and Immunohistochemistry. Not suitable for use in Immunocytochemistry or Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:40. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Western Blot: 1:200 for 1hr at RT with total cell lystates Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.