H7520-01
HS6ST1 (Heparan-sulfate 6-O-sulfotransferase 1, DKFZp547H098, FLJ25392, Heparan-sulfate 6-O-sulfotransferase 1, HS6ST, HS6ST-1, MGC116899, MGC116901)
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£832.00
- SKU:
- H7520-01
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to human HS6ST1 expressed in CHO cells.
- Format:
- Supplied as a lyophilized powder in PBS, 5% Trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extrracellular matrix.It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins,such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1,2).Heparan Sulfate consists of repeating residues of uronic acid and N-acetyl glucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-0 Sulfotransferase (HS2ST). The N-acetyl glucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spect rum of proteins differently. Among three HS6STs, HS6ST1is the first to be cloned(3).Mice deficient of the HS6ST1 homologue gene showed embryonic lethality(4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice