H7446-50B-ML550
Hrk (Activator of Apoptosis Harakiri, BH3-interacting Domain Containing Protein 3, BID3, DP5, Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain), Neuronal Death Protein DP5) (MaxLight 550)
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£882.00
- SKU:
- H7446-50B-ML550
- Anwendung:
- WB
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15aa near the center of human HRK (NP_003797).
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Weitere Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and hematopoietic cell apoptosis, in particular to growth factor deprivation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Detects a band at ~13kD in mouse pancreas tissue lysate. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice