H6202-42C
HMG4 (High Mobility Group 4 (HMG) Transcription Factor, HMG 4, HMG-4, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
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£761.00
- SKU:
- H6202-42C
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa1-180 from human HMGB3 expressed in E. coli (O15347).
- Klon:
- 11n84
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- High mobility group protein B3 is a 23kD nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200aa precursor, which is demethylated to produce the 199aa mature chain. The protein contains two HMG box DNA-binding domains aa9-79-93-161 and an acidic Asp/Glu-rich region aa181-200. Human HMGB3 shares 98aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single-stranded DNA and unwinds double stranded DNA. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice