H6098-39R

HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B + C, MHC HLA ABC)

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£338.00

SKU:: H6098-39R
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Klon:: W6/32
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. For antibody without azide, see H6098-39RX.
Weitere Details:: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells. HLA-ABC antigen is present on most nucleated cells of humans and old world monkeys. HLA Class 1 Antigen ABC is a monomorphic determinant of human Class I HLA molecules (HLA-A,B,C) associated with b2-microblogulin. It is expressed at the surface of human lymphoblastic B cells. Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C. HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD). The smaller beta chain bears homology with immunoglobulin molecules. The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus. This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues. HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor. HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells. Applications: Suitable for use in Blocking (6,7), Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation (2), Inhibition of NK cell-mediated lysis (10), and Activation (8,9). Other applications not tested. Recommended Dilutions: Immunofluorescence: ≤2ug/10e6 cells in 100ul volume or 100ul whole blood. Western Blot: Non-reducing (3) Immunohistochemistry: Acetone-fixed frozen sections (4,5) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice