H5109-50A-FITC
Histone Deacetylase 2, CT (HDAC2, Tanscriptional Regulator Homolog RPD3, HD2) (FITC)
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£1,042.00
- SKU:
- H5109-50A-FITC
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A synthetic 11 amino acid peptide derived from the C-terminus of the mouse HDAC2 protein. Species sequence homology: chicken single amino acid difference.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- Covalent modification of histone proteins plays an important role in the regulation of gene expression. In particular, acetylation of lysine residue on core histone proteins alters nucleosomal conformation and thereby regulates the accessibility of transcriptional regulatory proteins to chromatin templates. The causal relationship between histone acetylation and gene expression has been furthered by the identification of which posses intrinsic histone acetylase and deacetylase activity. Of the protein which posses intrinsic histone deacetylase activity, the best described are members of a common family who’s founding members were human HDAC1 and yeast Rpd3. HDAC1 is a 55kD protein which is 60% identical to the yeast global transcriptional regulator, Rpd3p. The ~50kD HDAC2 (also referred to as mRPD3) protein is 85% identical to HDAC1. HDAC2 was identified as a protein that interacts with the sequence-specific activator/repressor YY1. The HDAC proteins do not possess either a DNA-binding domain or a protein interaction domain. Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: FLISA: 0.1-1.0ug/ml Western Blot: 1-2ug/ml Immunoprecipitatioin (native): 5ug Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice