H2030-21
Hepsin (TMPRSS1)
Größe
£672.00
- SKU:
- H2030-21
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa241-260, GGYLPFRDPNSEENSNDIAL of human hepsin. Species Sequence Homology: Mouse and rat: 88% (17/20aa)
- Format:
- Supplied as a lyophilized powder from TBS, pH 7.4, 5mg/ml BSA. Reconstitute with 500ul sterile ddH2O.
- Weitere Details:
- Hepsin is a type II membrane-associated protein that has an extracellular proteolytic domain and exhibits low sequence homology to other known proteases (1,2). Hepsin overexpression is observed in the prostate, breast, kidney, and ovarian cancers and due to low homology to other known proteases may provide a unique target for pharmacological therapy (3-7). Hepsin is necessary for cell growth in vitro and may play a role in metastatic expansion for Factor VII activation (6,9). The observed molecular mass of hepsin by immunoblotting is 45.3kD. Cloning and characterization of murine and rat hepsin indicate 88% overall homology with human hepsin (10-12). Northern blotting reveals high levels of hepsin transcripts in liver (2,7). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:200 Immunohistochemistry: 1-2ug/ml; Formalin-fixed paraffin-embedded sections. Antigen retrieval results in higher background staining and is not recommended. Optimal dilutions to be determined by the researcher. Positive Control: K562 cell lysate Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice