H1831-25M
Heat Shock Cognate Protein 70 (HSC70, HSP73)
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£863.00
- SKU:
- H1831-25M
- Reinheit:
- Ascites
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Mouse spermatogenic cell protein.
- Klon:
- 6D447 (13D3)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Weitere Details:
- HSC70-interacting protein (Hip) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of the heat shock protein HSC70 in eukaryotic cells. Hip interacts with the ATPase domain of HSC70 molecules through the tetratricopeptide repeats and flanking charged a-helices of Hip. This binding reaction is dependent on HSP40 in the presence of ATP and becomes Hsp40-independent if HSC70 is preincubated with ADP. Upon binding to HsSC70, Hip stabilizes the ADP-bound state of HsSC70, a conformation that has a high affinity for unfolded substrate proteins. Hip by itself binds unfolded polypeptides, suggesting that it may possess chaperone activity. Together with HSP40, Hip may regulate the chaperone activity of HSC70 by stabilizing the chaperone- substrate complex. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Blocking/Neutralization and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 -1:5000 Immunohistochemistry (frozen and paraffin): 1:20-1:200 Flow Cytometry: 1:10 - 1:1000 Immunoprecipitation: 2ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice