G8957-04-BIOTIN
Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte protease, Protease Serine B, SECT) (Biotin)
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£837.00
- SKU:
- G8957-04-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to full length human Granzyme B.
- Klon:
- GB7
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:50-1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Positive Control: Granzyme B, is expressed within the granules of cytolytic lymphocytes (CTLs). Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Reagents: I1904-65W IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice