G8600-81C

G-Protein Coupled Receptor 77 (GPCR77, GPR77, C5L2, C5a Anaphylatoxin Chemotactic Receptor)

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£825.00

SKU:: G8600-81C
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: CHO cells transfected with mouse C5L2 Q8BW93.
Klon:: 9H130
Format:: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Weitere Details:: C5L2, also known as GPR77, is a 45kD 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% aa sequence identity with mouse and rat C5L2. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.