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G8600-40

G-Protein Coupled Receptor 34 (GPCR34, GPR34)

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£832.00

SKU:
G8600-40
Reinheit:
Purified by Protein G affinity chromatography from cell culture supernatant.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
NS0 cells transfected with human GPR34. Uniprot/Accession Number: Q9UPC5
Klon:
8H179
Format:
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:
GPR34 is a widely expressed 75-90kD seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% aa sequence identity with mouse and rat GPR34. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 A172 human glioblastoma cells fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml using immersion fixed A172 human glioblastoma cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Versandbedingungen:
Blue Ice