G8170-50G

Glycogen Synthase Kinase 3 beta (GSK3b)

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£512.00

SKU:: G8170-50G
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Klon:: 3A11
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Weitere Details:: Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3alpha and GSK-3beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility. Applications: • Western Blot • Enzyme-Linked-Immunosorbent-Assay (ELISA) Recommended Dilution: ELISA: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.