G8140-14B

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH, G3PD, MGC88685)

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£927.00

SKU:: G8140-14B
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Rabbit Muscle purified G3PDH.
Klon:: 7C33
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Weitere Details:: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH/GAPD/G3PD (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). G3PDH is a cytosolic enzyme. It is thought to be a tetramer of identical chains ~36kD. G3PDH (mouse/rat/rabbit 333; human 335-aa,chromosome 12p13.31-p13.1). The enzyme is present in such widely separated forms as man, lobster, and E. coli. Its rate of evolutionary change is one of the slowest known. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 3-6ug/ml using ECL technique. ELISA: 0.5-1ug/ml, Control antigen can be used to coat ELISA plates at 1ug/ml Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.