G3057-20G
Glucose Regulated Protein 78 (Hspa5, Heat Shock Protein 5, 78kD, 78kD Glucose-Regulated Protein precursor, AL022860, AU019543, Bip, BiP, D2Wsu141e, D2Wsu17e, Grp78, GRP 78, Heat shock 70kD protein 5, Heat Shock 70kD Protein 5, Hsce70, Immunoglobulin heavy chain-binding protein, mBiP, Sez7, SEZ-7)
Größe
£473.00
- SKU:
- G3057-20G
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse BiP:u chain complexes
- Klon:
- 5K23
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
- Weitere Details:
- The immunoglobulin heavy chain binding protein BiP (Binding Protein, [1]) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as “molecular chaperones,” which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (mu+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7 Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Studies of immunoglobulin assembly and secretion. Other applications not tested. Recommended Dilution: Western Blot: ≤5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice