G2035-14A

GITRL (Glucocorticoid Induced TNF-Related Ligand, AITRL, Activation-Inducible TNF-Related Ligand, TNFSF18)

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£442.00

SKU:: G2035-14A
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human GITRL expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2 Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Weitere Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Sandwich ELISA: 0.5-2ug/ml using 100ul/well. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human GITRL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 3ug/ml using A-431 cells incubated overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.