G2035-08V

GITR (Glucocorticoid-induced TNFR-related Protein, Activation-inducible TNFR Family Receptor, AITR, CD357, Tumor Necrosis Factor Receptor Superfamily Member 18, TNFRSF18, UNQ319/PRO364) (FITC)

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£639.00

SKU:: G2035-08V
Reinheit:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to human GITR, PHA stimulated human PBL
Klon:: 11K8
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Weitere Details:: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or use 1.6ul of concentrated stock per 5x10e5 cell test). Optimal dilutions to be determined by the researcher. Fluorescein/IgG molar ratio: 8.8. Flow Cytometry Protocol: 1. Ficoll prepared human peripheral blood mononuclear cells with stimulated for 5 days with PHA. 2. Five x 10e5 cultured human peripheral blood mononuclear cells were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG. 3. Incubate 45 minutes on ice with 80ul of G2035-08V at a 1:50 dilution factor (10ug/ml). 4. Cells were washed three times, fixed and analyzed by FACS. 5. Cells stained positive with a mean shift of 0.85log10 fluorescent units when compared to a Mouse IgG3/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice