G1044-51
Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2)
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£800.00
- SKU:
- G1044-51
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant human Galectin 3. Epitope: aa1-58 of Galectin 3.
- Klon:
- 4i288 (A3A12)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Weitere Details:
- Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an imjportant region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot,Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:2000 Flow Cytometry: 1:100-1:2000 Immunohistochemistry: 1:10-1:500 Immunofluorescence (IC):1:10-1:500 Immunohistochemistry: (Paraffin): 6ug/ml Immunohistochemistry: (Frozen): 6ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Rat Olfactory bulb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice