G1017-19H
GADD45A, Recombinant, Human (Growth Arrest and DNA Damage-inducible Protein GADD45 alpha, DNA Damage-inducible Transcript 1 Protein, DDIT1, DDIT-1, GADD45)
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£320.00
- SKU:
- G1017-19H
- Anwendung:
- WB
- Molekulargewicht:
- 19.4
- Reinheit:
- ~ 85% (SDS-PAGE). Purified by using conventional chromatography techniques.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris HCl, pH 8.0, 0.1M sodium chloride, 10% glycerol.
- Weitere Details:
- GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E. coli. Recombinant corresponding to aa1-165 of human GADD45A 6x His tagged expressed in E. coli. AA Sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice