G1009-02A
G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a, Protein G9a )
Größe
£793.00
- SKU:
- G1009-02A
- Anwendung:
- WB
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to G9a (BAT8). Cellular Localization: Nucleus. Species sequence homology: rat and canine.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25-2ug/ml detected G9a (BAT8) on 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice