Fz5 (FZD5, Frizzled Homolog 5 (Drosophila), C2orf31, Chromosome 2 Open Reading Frame 31, Frizzled-5, Frizzled 5 precursor, Frizzled-5 precursor, Fz-5, FzE5, hFz5, Hfz5, HFZ5, Uncharacterized Protein C2orf31)

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£793.00

SKU:: F9900
Anwendung:: WB
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa564-585, C-TGRTPPGPAATYHKQVSLSHV, of human Fz5, conjugated to KLH. Epitope: C-terminus.
Format:: Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide, 30% glycerol.
Weitere Details:: The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), beta- and gamma- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Lysates from mouse liver, C2C12, Jurkat and HUVEC cells were resolved by electrophoresis, transferred to PVDF and probed with F9900. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Control: Mouse liver, C2C12, Jurkat and HUVEC cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice