F9500-03G

Fyn (C-syn Proto-oncogene, FYN Oncogene Related to SRC FGR YES, OKT3-induced Calcium Influx Regulator, p59 FYN, Proto-oncogene c-Fyn, Proto-oncogene Syn, Src-like Kinase, SLK, SYN, Tyrosine Kinase p59fyn (T), Tyrosine-protein Kinase Fyn)

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£741.00

SKU:: F9500-03G
Reinheit:: Purified by Protein A affinity chromatography from hybridoma culture supernatant. (>95% SDS-PAGE).
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Bacterially expressed recombinant protein fragment corresponding to aa7-176 from human Fyn.
Klon:: FYN-1
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Weitere Details:: Fyn is a non receptor protein tyrosine kinase (~59kD) that belongs to the Src family of cytoplasmic tyrosine kinases. Applications: Suitable for use in Immunoprecipitation, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunoprecipitation: Prepare cell lysates in lysing buffer with N-dodecyl beta-D-maltoside (20mM Tris/Cl, 100mM NaCl pH 8.2, 1% laurylmaltosid (w/v), 50mM NaF) for 30 min on ice in an orbital incubator in lysing buffer. Western Blot: 1:500 Optimal dilutions to be determined by the researcher. WB Positive Control: Jurkat cell line IP Positive control: J77 T-cell line lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.