F9200-48
Fucosyltransferase 3 (FUT3, Galactoside 3(4)-L-fucosyltransferase, Blood Group Lewis alpha-4-Fucosyltransferase, Lewis FT)
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£761.00
- SKU:
- F9200-48
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg35-Thr361 of human Fucosyltransferase 3 expressed in NSO cells (P21217). Species Sequence Homology: Chimapanzee FUT5, 99%; chicken, 52%.
- Klon:
- 8H187
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha-4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa1-15), a transmembrane region (aa16-34) and an ectodomain (aa35-361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well. Western Blot: 1ug/ml. Using a colormetric detection system, the detection limit is ~20ng/lane under non-reducing and reducing conditions. Immunoprecipitation: 25ug/ml immunoprecipitates rhFUT3 or rhFUT5 from conditioned cell culture medium. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice