FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

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£626.00

SKU:: F9050-10K
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a region between aa400-431 from the C-terminal region of human FOXP3.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Weitere Details:: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 detects a band at ~47.2kD Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry: 1:50-1:1000. Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse. Immunohistochemistry (Paraffin): 1:200-1:1000 Immunohistochemistry (Frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice