F7000-05D
FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)
Größe
£787.00
- SKU:
- F7000-05D
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asn121-Asn449 from human FRS2 expressed in E. coli. Species Sequence Homology: canine - 99%, mouse - 94%.
- Klon:
- 8B20
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- FRS2 (FGFR substrate 2; also SNT and FSR2-a) is a 70-90kD member of the FSR family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors, and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids in length. It contains a membrane-anchoring myristoylation signal (aa1-6), a PTB domain that interacts with FGF and NGF receptors (aa13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa196-471). One splice form shows a deletion of the C-terminal four amino acids. Over aa121-449, human FRS2 is 99% and 94% identical to dog and mouse FRS2, respectively. Applications: Suitable for use in Direct ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. Validated using A172 human glioblastoma cell line cells fixed with paraformaldehyde and permeabilized with saponin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice