F4305-18D
Fibroblast Growth Factor Receptor 3 (FGFR3)
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£473.00
- SKU:
- F4305-18D
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa792-806 (K-DLLPPAPPSSGG- SRT) of the cytoplasmic region of human FGFR3 with N-terminal added lysine (KLH, glutaraldehyde). Cellular Localization: Plasma membrane; Single-pass type I membrane protein
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.
- Weitere Details:
- Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000; 293T cells were transfected with FGFR3 plasmide. NBT/BCIP was the substrate. Band at ~110-120kD. Immunohistochemistry (Formalin-fixed paraffin): 1:250-1:500 using Tyrpsin-digested sections. Stains human placenta and umbilical cord. Staining is observed in amniotic epithelium, tubules and squamous epithelium. Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysates of transfected 293T (embryonic kidney) cells expressing recombinant human FGFR3. Recommended Secondary Antibodies (WB, IP): I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb Recommended Secondary Antibodies (IHC): I1904-40N: IgG, Fc, X-Adsorbed (Biotin) Pab Gt x Rb I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice