F4212-93D
Fibroblast Growth Factor 21, aa29-209, Recombinant, Human (FGF-21)
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£422.00
- SKU:
- F4212-93D
- Molekulargewicht:
- 21.6
- Reinheit:
- ≥90% (SDS-PAGE) Endotoxin: ≤1EU/1ug (LAL)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.
- Weitere Details:
- FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. Recombinant protein corresponding to aa29-209 frin human FGF-21, fused to His-Tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS Biological Activity: The ED50 for this effect is < 12 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 83,000 units/ml). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice