F4203-03C
Fibrinogen, Citrullinated (FGA, FGB, FGG, Fibrinogen A alpha Polypeptide Chain, Fibrinogen alpha Chain, Fibrinogen beta Chain, Fibrinogen gamma Chain)
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£1,374.00
- SKU:
- F4203-03C
- Reinheit:
- Purified.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Deiminated mouse fibrinogen peptide.
- Klon:
- 3D1
- Format:
- Supplied as a lyophilized powder from 10mM ammonium bicarbonate buffer, 2mg BSA. Reconstitute with 200ul 100mM PBS or Tris-HCl, pH 7-8.
- Weitere Details:
- Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:2000-1:4000 Immunohistochemistry: 1:100-1:500 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C Reconstitute with 100mM PBS or Tris-HCL, pH 7-8. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice