F4194
Fibrillin 1
Größe
£825.00
- SKU:
- F4194
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine zonular fibrils
- Klon:
- 11C1.3
- Format:
- Supplied as a liquid.
- Weitere Details:
- Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome. Applications: Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: reacts with 350kD protein released from bovine aortic smooth muscle cells, which is identical to that of fibrillin. Immunohistochemistry (paraffin): 1:200-1:500. Paraffin sections reactive after microwave citrate buffer antigen retrieval. Immunoprecipitation: 5 microliters of antibody diluted in no more than 400 microliters of concentrated supernatants from bovine smooth muscle cells. It is recommended to use either an anti-mouse IgG bead or a rabbit anti-mouse capture antibody followed by protein A. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice