F4150-20
FK506 Binding Protein 8 (FKBP8, FK506 Binding Protein 38kD, FKBP38, Rotamase)
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£825.00
- SKU:
- F4150-20
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met58-Gly382 from human FKBP38, expressed in E. coli.
- Klon:
- 7H190
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- FK506 Binding Proteins (FKBPs) are intracellular receptors for the immuno-suppressive drug FK506. The FKBP/FK506 complex exerts its immunosuppressive effects by inhibiting calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase that functions as a critical signaling molecule during T-cell activation. FKBP38, also known as FKBP8, is a 355aa protein with a calculated molecular weight of 38.7kD and an apparent molecular mass of ~60-64kD in SDS-PAGE. FKBP38 binds to and inhibits calcineurin even in the absence of FK506, indicating that FKBP38 is a constitutively active inhibitor of calcineurin. Additionally, FKBP38 co-immunoprecipitates with Bcl-2 and Bcl-xL, suggesting that FKBP38 may regulate apoptosis by anchoring Bcl-2 and Bcl-xL to mitochondrial membranes. Mouse FKBP38 shares 94% and 87% sequence identity with human and rat FKBP38, respectively. Applications: Suitable for use in Western Blot. Other applications no tested. Recommended Dilution: Western Blot: 0.5ug/ml using HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line and L6 rat myoblast cell line Optimal dilution to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice